Science World Journal of Biomedical Research and Reviews

Editor Name: Xagorari Angeliki

Country: Greece

University: Democritus University of Thrace

Educational Qualification: Ph.D in Molecular Biology

Department: Department of Genetics and Molecular Biology

Email: xagorariangeliki@yahoo.com

Research Interest: Molecular Biology and Biotechnology

BIOGRAPHY:

RESEARCH EXPERIENCE:

ï‚§ 1986-87, Genetic study for the identification of a hybrid fish from parental species Leuciscus and Chalcarburnus, under the supervision of Prof Triantafillidis, Lab. of Genetics, Biology Department, Aristotelian University of Thessaloniki

ï‚§ Jan. 1990- April 1990, Screening for the isolation of the TAG gene encoding for a neuroprotein under the supervision of Prof Papamatheakis, Lab. of Molecular Biology, Biology Department, Institute of Molecular Biology and Biotechnology (IMBB), Heraklion, Crete, Greece

ï‚§ April 1990- July 1990, Screening for the isolation of the tRNA gene Saccharomyces cerevisiae, under the supervision of Dr Alexandraki, Lab. of Yeast Genetic, Biology Department, Institute of Molecular Biology and Biotechnology (IMBB), Heraklion, Crete, Greece

ï‚§ Sept. 1990- Dec. 1990, Research on the subpopulation of T lymphocytes derived from different embryonal mice imbreds under the supervision of Prof Athanasakis-Vasiliadou, Lab. of Immunology, Biology Department, University of Crete, Heraklion, Crete, Greece

ï‚§ April 1991- Dec. 1996, Ph.D thesis. Title: Molecular characterisation of the region 5q23-32: identification of two novel genes, under the supervision of Prof Anagnou, Lab. of Molecular Biology, Biology Department, University of Crete and Institute of Molecular Biology and Biotechnology (IMBB), Heraklion, Crete, Greece

ï‚§ Jan. 1997- Mar. 1988: postdoctoral position in Dr Meisterernst Lab. Study on the human transcriptional cofactor NC2 and PC4 in C. elegans, Genzentrum, LMU, Munich, Germany

ï‚§ May 1988- Jan. 1999: postdoctoral position in Dr Zorbas Lab. Study of the signal transduction mechanism of inteferon Î± and Î² on regulatory factor IRF-1, Genzentrum, LMU, Munich, Germany

ï‚§ Jan 1999-Jan. 2002: postdoctoral position. Study of the effects of tyrosine kinase inhibitors on signal transduction pathways in endotoxin-activated macrophages, Lab. of Prof Roussos in collaboration with Dr Papapetropoulos, Medical School, University of Athens, Athens, Greece

ï‚§ Jan 2002-Dec 2010: Gene Therapy Laboratory, Hematology Department/BMT Unit, G.Papanikolaou General Hospital.

ï‚§ Current position: Head of Laboratory of Public Cord Blood Bank /Hmeatology Unit/BMT, G.H.Papanicolaou, Thessaloniki, Greece

TEACHING EXPERIENCE:

â€¢ 1989-1990: Lectures in Biochemistry, Immunology, Parasitology to students in applied microbiology, Pasteur School, Thessaloniki

â€¢ 1991-1992, 1993-1994: Laboratory courses in Microbiology, Biology Department, Heraklion, Crete, Greece

â€¢ 1999-2000, 2000-2001: Lectures in Parasitology, Department of Public Health, Tecnological Educational Institutes (TEI), Athens

â€¢ 2005, Lectures in Microbiology, Virology, Department of Molecular Biology and Genetics, Democritus University, Alexandroupolis, Thrace.

PUBLICATIONS:

1. Xagorari A. and Anagnou N.P. (1997). Simplified and rapid approach for the selection of cDNAs encoded by YACs.Trends in Genetics , 13 (2): 82

2. Xagorari A., Papapetropoulos A., Mauromatis A., Economou M., Theodore Fotsis and Roussos C.(2001). Luteolin inhibits an endotoxin-stimulated phosphorylation cascade and pro-inflammatory cytokine production in macrophages. J. Pharmacol. Exp. Ther., 296 (1):181-187

3. Kosmidou I., Xagorari A., Roussos C. and Papapetropoulos A. (2001). Reactive oxygen species stimulate VEGF production from C2C12 skeletal myotubes through a PI3-K/AÎºt pathway. AJP: Lung cellular and molecular physiology, 280: L585-L592

4. Kotanidou A., Xagorari A., Bagli E., Îšitsanta O., Fotsis T., Papapetropoulos A., and Roussos C.(2002) Luteolin reduces LPS-induced lethal toxicity and expression of pro-inflammatory molecules in mice. Am. J Res. and Critical Care Medicine, 165: 1-6

5. Kosmidou I., Vassilakopoulos T., Xagorari A., Zakynthinos S., Papapetropoulos A. and Roussos C. (2002) Production of interleukin-6 by skeletal myotubes: Role of reactive oxygen species. Am. Rev. of Res. Cell and Mol. Biology, 26:1-7

6. Xagorari A., Roussos C., and Papapetropoulos A. (2002) Inhibition of LPS-stimulated pathways in macrophages by the flavonoid luteolin. Brit.J Pharmacol.,136, 1058:1064

7. Yannaki Î•., Athanasiou E., Xagorari Î‘., Konstantinou V., Batsis I., Anagnostopoulos, A., Fassas A. (2005) G-CSF-primed hematopoietic stem cells accelerate recovery from acute and chronic carbon tetrachloride (CCL4)-induced liver injury. Exp. Hematology, 33, 108-119

8. Yannaki E, Anagnostopoulos A, Kapetanos D, Xagorari A, Iordanidis F, Batsis I, Kaloyannidis P, Athanasiou E, Dourvas G, Kitis G, Fassas A (2006)Lasting amelioration in the clinical course of decompensated alcoholic cirrhosis with boost infusions of mobilized peripheral blood stem cells. Exp. Hematology, 34(11):1583-7.

9. Xagorari A, Chlichlia K. (2008) Toll-like receptors and viruses: induction of innate antiviral immune responses. Open Microbiol J,;2:49-59.Â

10. Tsingotjidou AS, Emmanouilides CE, Siotou E, Poutahidis T, Xagorari A, Loukopoulos P, Sotiropoulos D, Bekiari C, Doulberis M, Givissis P, Fassas A, Anagnostopoulos A. (2009) Establishment of an animal model for WaldenstrÃ¶m's macroglobulinemia. Exp. Hematology, 37(4):469-76.

11. E.Yannaki, T. Papayannopoulou, E. Jonlin, F. Zervou, G. Karponi, A. Xagorari, P. Becker, N. Psatha, I. Batsis,Â P. Kaloyannidis,V. Tahynopoulou, V. Constantinou, M. Bouida â€¢ K. Kotta, A. Athanassiadou, A. Anagnostopoulos, A. Fassas â€¢ G. Stamatoyannopoulos. Hematopoietic stem cell mobilization for gene therapy of adult patients with severe Î²-thalassemia: results of clinical trials using G-CSF or plerixafor in splenectomized and nonsplenectomized subjects. Molecular Therapy 20 (1): 230-8.Â

12. Xagorari A., Siotou E., Yiangou M., Tsolaki E., Bougiouklis D., Sakkas L., Fassas A., Anagnostopoulos A. 2013. Protective effect of mesenchymal stem cell-conditioned medium on hepatic cell apoptosis after acute liver injury. International journal of clinical and experimental pathology 6 (5): 831-40.

PRESENTATIONS/POSTERS IN $! CONFERENCES (12 AWARDS)Â

SELECTED ABSTRACTS IN CONFERENCES:

1. A Xagorari, M Iskas, D Bougiouklis, E Gavrilaki, G Karavalakis, D Papadopoulou, D Papaioannou, I Batsis, D Mallouri, I Sakellari, A. Anagnostopoulos, DÂ Sotiropoulos. Identification and evaluation of CD34+microparticles in grafts and patients post allogeneic hematopoietic cell transplantation. 44th EBMT, Lisbon, Portugal, March 18-21,2018

2. A Xagorari, M Geroussi, A Sioga, D Bougiouklis, A Argiriou, A Anagnostopoulos, and D Sotiropoulos . miRNA detection of stem cell specific microparticles of cord blood.1st Greco-Israeli Hematology Meeting, Thessaloniki, April 26-28, 2018.

3. A Xagorari, M Iskas, D Bougiouklis, A Bouinta,Â G Karavalakis, D Mallouri, I Sakellari, A Anagnostopoulos, D Sotiropoulos. Identification of CD34+,CD33+,CD3+ and CD19+ microparticles in peripheral blood of patients undergoing allogeneic stem cell transplantation. 1st Greco-Israeli Hematology Meeting, Thessaloniki, April 26-28, 2018.

4. A.Xagorari, M. Iskas, MD, D. Bougiouklis, E. Apostolidou, G.Karavalakis,V. Kalaitzidou, Despina Mallouri, MD2*, I. Sakellari, A. Anagnostopoulosand D. Sotiropoulos. Comparative Evaluation of CD34+/CD33+/CD3+/CD19+ Microparticles in Peripheral Blood of Patients during Allogeneic Stem Cell Transplantation. 59th Annual Meeting of American Society of Hematology (Poster presentation)

5. A.Xagorari, M. Geroussi, A. Marvaki, M. Samalidou, A. Sioga, D. Bougiouklis, Anagnostis Argiriou, Achilles AnagnostopoulosÂ and D. Sotiropoulos, Detection of microRNAs in CD34+ Microparticles ofÂ Cord Blood Units, 3-5 ÎÎ¿vember 2016 (awarded) .

6. A. Xagorari, M. Geroussi, A. Marvaki, M. Samalidou, A. Sioga, D. Bougiouklis, Anagnostis Argiriou, Achilles AnagnostopoulosÂ and D. Sotiropoulos, Detection of microRNAs in CD34+ Microparticles ofÂ Cord Blood Units. 58th Annual Meeting of American Society of Hematology (Oral presentation), San Diego, Dec 3-6, 2016 Blood 2015; 128(22):820

7. A.Xagorari, A. Syrigou, Z. Bousiou, P. Karavassilidou, M. Keramari, M. Ganidou, A. Anagnostopoulos, D. Sotiropoulos. Surveillance study of infectious diseases in umbilical cord blood and maternal samples of Public Cord Blood Bank of Thessaloniki (TSCB) Greece. ECCMID Amsterdam April 9-12, 2016.

8. E. Gounari, A. Papadopoulou, M. Yiangou, A. Xagorari, A. Anagnostopoulos, E. Yannaki, P. Kaloyannidis. An optimized ex vivo logâ€”scale expansion of UCB-derived myeloid Dcs, tpwards efficient cancer immunotherapy. 55th ASH, December 6-9, 2014, San Francisco, USA USA. Blood, Vol. 122, 21, November 15 , 2013.

9. A.Xagorari, S. Sarakatsani, I. Karvounidou, K. Kotta, Z. Moustakoudaki, A. Spiridonidou, D. Sahpazidou, m Gkanidou, A. Anagnostopoulos, D. Sotiropoulos. Cord Blood Bank activity in Thessaloniki Cord Blood Bank (TSCB) Greece:4 years data experience. XXXV World Congress of the International Society of Hematology China, September 4-7, 2014.

10. E. Gounari, A. Papadopoulou, N. Zogas, N. Psatha, G. Karponi, F. Zervou, D. Bougiouklis, A. Xagorari, M. Yiangou, A. Anagnostopoulos, E. Yannaki, P. Kaloyannidis. Large-Scale Myeloid Dendritic generation from umbilical CD34+ cells using G-Rex Bioreactors. EBMT, 2014, Milan, Italy.

11. A.Xagorari, M.Samalidou, D. Bougiouklis, D.Zacharaki, N. Leventopoulou, A. Sioga, L. Economou, D. Sotiropoulos, A. Anagnostopoulos. Detection ofÂ microparticles in cord blood units.Â EBMT 2013, London, UK.

12. Bone Marrow Transplantation Vol. 48, Supplement 2,Â 2013, S109.Â

13. Î‘. Î‘lataki, A. Tiptiri-Kourpeti, M. Koureta, P. Ypsilantis, C. Simopoulos, A. Xagorari, A. Îšaufmann, K. Chlichlia. DNA Vaccines targeting surviving inhibit growth mammary adenocarcinoma in syngeneic mice. Proceedings of the 35th Scientific Conference of Hellenic Association for Biological Sciences. Nafplio, May 23-25, 2013.Â

14. A. Xagorari, M. Samalidou, D. Bougiouklis, K. Kotta, A. Sioga, L. Economou, A. Anagnostopoulos, D. Sotiropoulos. Identification of CD34+ Microparticles In Cord Blood Units. 55th ASH, December 7-10, 2013 Îew Orleans, USA. Blood, Vol. 122, 21, November 15 , 2013.

15. A.Xagorari, E. Siotou, M. Yiangou,Â E. Tsolaki,, D. Bougiouklis, L. Sakkas,Â Athanassios Fassas, A. Anagnostopoulos. Mesenchymal stem cell-conditioned medium attenuates hepatic cell apoptosis after acute liver injury. 22nd Î•uropean Tissue Repair Society Meeting, 4-5 October, 2012, Î‘thens, Greece.

16. E.Yannaki, T. Papayannopoulou, E. Jonlin, F. Zervou, G. Karponi, A. Xagorari, P. Becker, N. Psatha, I. Batsis, P. Kaloyannidis, V. Tahynopoulou, V. Constantinou, A. Bouinta, K. Kotta, A. Athanassiadou, A. Anagnostopoulos, A. Fassas. Hematopoietic cell mobilization for gene therapy of adult patiets with severe beta-thalassemia: the challenge of splenectomy and the role of plerixafor. 53rd ASH meeting, San Diego, 10-13 December 2011.

17. A.Xagorari, E. Siotou, M. Yiangou, E. Yannaki, D. Bougiouklis, L. Sakkas, A. Fassas, A. Anagnostopoulos. The effect of mesenchymal stem cell conditioned medium on apoptosis of primary hepatocytes after acute liver injury. Inflammation and Disease. Lisbon, Portugal 26-28 September 2010.Â

18. Yannaki, E., Athanasiou E., Xagorari, A., Constantinou, V., Batsis, I., Fassas, A., Anagnostopoulos, A. Transplantation of mobilized peripheral blood stem cells in unconditioned hosts results in formation of donor-derived hepatocytes in an experimental liver injury model. Panhellenic Hematological Conference, Alexandroupolis, 2003 (1st price) and 45th American Society of Hematology, San Diego, 2003.

19. Xagorari A., Roussos C., Papapetropoulos A. Molecular targets of luteolin (lut) in RAW 264.7 in murine macrophages. 5th State of the Art. Interdisciplinary Review Course. Athens. April 2001 (1st price).

20. Xagorari A., Papapetropoulos A., Fotsis T. and Roussos C. Study on the inhibitory mechanism of the intracellular LPS signaling pathway in mice macrophages using the flavonoid luteolin. Panhellenic Biological Conference, Molecular Biology-Cytogenetics in Health, Athens, 2000.

21. Xagorari A., Roussos C., Papapetropoulos A. Luteolin inhibits LPS-signaling in vivo and in vitro. Vascular Endothelium: Source and Target of Inflammatory mediators. NATO Advanced Study Institute, Crete 2000.

22. Kosmidou I., Vasilakopoulos T., Xagorari A., Papapetropoulos A., Zakynthinos S.,Â Roussos C. Reactive oxygen species induce secretion of VEGF from skeletal muscle. NATO Advanced Study Institute, Crete 2000 and World Congress for Lung Health and 10th ERS Annual Congress, Florence, Italy, Sept. 2000.

23. Xagorari A , Raggousis I., Anagnou N.P. Functional study on the critical region 5q-. Isolation of a new gene. Understanding eucaryotic gene expression. Sweden, June 1996

PUBLISHED ABSTRACTS IN CONFERENCES:

1. Ioanna Kosmidou, Theodoros Vasilakopoulos Angeliki Xagorari, Charis Roussos and Andreas Papapetropoulos. Reactive oxygen species induce secretion of VEGF from skeletal myocytes. ERS meeting, Florence 2000; Eur. Respir. J., 16,Sup. 31:180s

2. Ioanna Kosmidou, Theodoros Vasilakopoulos, Angeliki Xagorari, Spyros Zakynthinos, Charis Roussos and Andreas Papapetropoulos (2000). Oxidative Stress inducesÂ IL-6 production in cultured skeletal myocytes. Am. J. Resp. Crit. Care Med. 161:A199, 2000

3. E.Yannaki, E. Athanasiou, A. Xagorari, V. Konstantinou, I. Batsis, A. Fassas, A. Anagnostopoulos. Transplantation of mobilized peripheral blood stem cells in unconditioned hosts results in formation of donor-derived hepatocytes in an experimental liver injury model. 45th American Society of Hematology, Blood 2003, 102 (11):336Î±

4. E.Yannaki, E. Athanasiou, A. Xagorari, V. Konstantinou, I. Batsis, A. Anagnostopoulos, A. Fassas. G-CSF-mobilized hematopoietic stem cells accelerate recovery from acute and chronic carbon tetrachloride (CCL4)-induced liver injury. 45th American Society of Hematology, Blood 2003, 102 (11):336-337Î±

5. Bagli E., Xagorari A., Papapetropoulos A., Murphy C., Fotsis T. (2004) Angiogenesis in Inflammation. Autoimmunity Reviews 3 Suppl. 1 S7-S73.